The Perkin Elmer Opera Phenix automated confocal microscope is a very fast, sensitive, high throughput confocal microscope for imaging experiments on live or fixed cells and microtissues. Automated 3D imaging is possible of cell spheoroids, muscle fibers or small model organisms like Zebrafish. The Phenix is capable of 400,000 images a day and is equipped with a five laser system optimized for FRET applications, 20x, 40x and 63x water objectives with 1.25x to 20x air objectives. Slides to 1536 well plates can be imaged with 384 well plates being the standard. The four large format sCMOS camera setup is equipped with optics for simultaneous acquisition in up to four channels. Experiments of many plates can be automated with the robotic arm, plate storage and automated incubator. Images can be analyzed online with our Columbus Image Analysis web server.
If interested in running experiments on the Opera Phenix and for help with assay development, please contact Stephen Baird (sbaird@mgcheo.med.uOttawa.ca), Manager of the High Throughput Screening (HTS) Core Facility.
